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sgrna scaffold vector  (Addgene inc)


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    Structured Review

    Addgene inc sgrna scaffold vector
    Sgrna Scaffold Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+vector/pGL3-U6-sgRNA-PGK-puromycin+(Plasmid+%2351133)/pm41904535-60-1-5
    Average 94 stars, based on 166 article reviews
    sgrna scaffold vector - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Systemic gene therapy rescues retinal dysfunction and hearing loss in a model of Norrie disease.
    Article Snippet: .. M50 Super 8× TopFlash in pTA-Luc vector (12456) and M51 Super 8× FopFlash in pGL3 vector (12457) were obtained from Addgene. ..

    Article Title: Naringin Mitigates Chondrocyte Apoptosis in Osteoarthritis by Suppressing the miR-29a-3p-Bax Pathway.
    Article Snippet: The present study aims to explore potential therapeutic effects of Naringin on osteoarthritis (OA) and investigate the underlying mechanism.. The chondrocytes in the joint usually undergo detrimental changes during OA progression, including increased apoptosis. miRNAs emerge as crucial regulators in this processes.. The study delves into the intricate interplay between miR‐29a‐3p, BAX‐mediated apoptosis, and Naringin intervention.

    Article Title: m 5 C methylated lncRncr3 –MeCP2 interaction restricts miR124a- initiated neurogenesis
    Article Snippet: The beads were pelleted by magnet and the supernatant subjected to protein size-separation in 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels (Bio-Rad) and used for western blot experiments. .. To express appropriate sgRNAs in NE-4C cells for deletion of Rncr3 exons 2/3 or for Rncr3 full length KO, the pGL3 vector (Supplementary Table ) with the U6 promoter was used and co-transfected with pCAS9 into cells. pGL3-U6-sgRNA-PGK-puromycin was a gift from Xingxu Huang (Addgene plasmid # 51133; http://n2t.net/addgene:51133 ; RRID: Addgene_51133) and pCas9_GFP was a gift from Kiran Musunuru (Addgene plasmid # 44719; http://n2t.net/addgene:44719 ; RRID: Addgene_44719). .. For in vivo RNA pull-down assays and MeCP2-eGFP RIP experiments, cells were transfected with plasmids expressing eGFP fused with wild-type (pEGFP-N1_MeCP2(WT), gift from Adrian Bird ; Addgene plasmid # 110186; http://n2t.net/addgene:110186 ; RRID: Addgene_110186) or other MeCP2 mutants (Supplementary Tables and ).

    Article Title: Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids.
    Article Snippet: .. Plasmids: To express appropriate sgRNAs in human iPS cells for deletion of the HAT domain of hGCN5, the pGL3 vector with the U6 promoter was used and co-transfected with pCAS9 into cells. pGL3-U6-sgRNA-PGKpuromycin was a gift from Xingxu Huang[79] (Addgene plasmid # 51133; http://n2t.net/addgene:51133; RRID: Addgene_51133) and pCas9_GFP was a gift from Kiran Musunuru[80] (Addgene plasmid # 44719; http: //n2t.net/addgene:44719; RRID: Addgene_44719). ..

    Article Title: Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids
    Article Snippet: .. To express appropriate sgRNAs in human iPS cells for deletion of the HAT domain of hGCN5 , the pGL3 vector with the U6 promoter was used and co‐transfected with pCAS9 into cells. pGL3‐U6‐sgRNA‐PGK‐puromycin was a gift from Xingxu Huang [ ] (Addgene plasmid # 51133; http://n2t.net/addgene:51133; RRID: Addgene_51133) and pCas9_GFP was a gift from Kiran Musunuru [ ] (Addgene plasmid # 44719; http://n2t.net/addgene:44719; RRID: Addgene_44719). ..

    Article Title: Bone Metastatic Progression of Prostate Cancer is Regulated by TRIM28-LDHA Mediated Metabolism
    Article Snippet: LDHA promoter luciferase reporter assays were conducted according to the manual of Dual-Luciferase Reporter Assay System from Promega. .. Briefly, C4–2B cells were seeded in a 24-well plate and co-transfected with the Renilla expression plasmid pRL-TK (RRID:Addgene_27163) and the reporter constructs for LDHA promoter in pGL3 vector (RRID:Addgene_212937). ..

    Article Title: Nuclear GRP78 Promotes Metabolic Reprogramming and Therapeutic Resistance in Pancreatic Ductal Adenocarcinoma
    Article Snippet: Purpose: Stromal fibrosis limits nutritional supply and disarrays metabolism in pancreatic cancer (PDA, pancreatic ductal adenocarcinoma).. Understanding of themolecular basis underlyingmetabolic cues would improve PDA management.. The current study determined the interactionbetweenglucose-regulatedproteins78(GRP78) and hypoxia-inducible factor 1a (HIF-1a) and its mechanistic roles underlying PDA response to oxygen and glucose restrains.

    Article Title: Oncogenic KRAS Drives Lipofibrogenesis to Promote Angiogenesis and Colon Cancer Progression
    Article Snippet: DECEMBER 2023 CANCER DISCOVERY | 2653

    Reporter Assay:

    Article Title: Naringin Mitigates Chondrocyte Apoptosis in Osteoarthritis by Suppressing the miR-29a-3p-Bax Pathway.
    Article Snippet: The present study aims to explore potential therapeutic effects of Naringin on osteoarthritis (OA) and investigate the underlying mechanism.. The chondrocytes in the joint usually undergo detrimental changes during OA progression, including increased apoptosis. miRNAs emerge as crucial regulators in this processes.. The study delves into the intricate interplay between miR‐29a‐3p, BAX‐mediated apoptosis, and Naringin intervention.

    Article Title: Oncogenic KRAS Drives Lipofibrogenesis to Promote Angiogenesis and Colon Cancer Progression
    Article Snippet: DECEMBER 2023 CANCER DISCOVERY | 2653

    Mutagenesis:

    Article Title: Naringin Mitigates Chondrocyte Apoptosis in Osteoarthritis by Suppressing the miR-29a-3p-Bax Pathway.
    Article Snippet: The present study aims to explore potential therapeutic effects of Naringin on osteoarthritis (OA) and investigate the underlying mechanism.. The chondrocytes in the joint usually undergo detrimental changes during OA progression, including increased apoptosis. miRNAs emerge as crucial regulators in this processes.. The study delves into the intricate interplay between miR‐29a‐3p, BAX‐mediated apoptosis, and Naringin intervention.

    Luciferase:

    Article Title: Naringin Mitigates Chondrocyte Apoptosis in Osteoarthritis by Suppressing the miR-29a-3p-Bax Pathway.
    Article Snippet: The present study aims to explore potential therapeutic effects of Naringin on osteoarthritis (OA) and investigate the underlying mechanism.. The chondrocytes in the joint usually undergo detrimental changes during OA progression, including increased apoptosis. miRNAs emerge as crucial regulators in this processes.. The study delves into the intricate interplay between miR‐29a‐3p, BAX‐mediated apoptosis, and Naringin intervention.

    Article Title: Oncogenic KRAS Drives Lipofibrogenesis to Promote Angiogenesis and Colon Cancer Progression
    Article Snippet: DECEMBER 2023 CANCER DISCOVERY | 2653

    HAT Assay:

    Article Title: Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids.
    Article Snippet: .. Plasmids: To express appropriate sgRNAs in human iPS cells for deletion of the HAT domain of hGCN5, the pGL3 vector with the U6 promoter was used and co-transfected with pCAS9 into cells. pGL3-U6-sgRNA-PGKpuromycin was a gift from Xingxu Huang[79] (Addgene plasmid # 51133; http://n2t.net/addgene:51133; RRID: Addgene_51133) and pCas9_GFP was a gift from Kiran Musunuru[80] (Addgene plasmid # 44719; http: //n2t.net/addgene:44719; RRID: Addgene_44719). ..

    Article Title: Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids
    Article Snippet: .. To express appropriate sgRNAs in human iPS cells for deletion of the HAT domain of hGCN5 , the pGL3 vector with the U6 promoter was used and co‐transfected with pCAS9 into cells. pGL3‐U6‐sgRNA‐PGK‐puromycin was a gift from Xingxu Huang [ ] (Addgene plasmid # 51133; http://n2t.net/addgene:51133; RRID: Addgene_51133) and pCas9_GFP was a gift from Kiran Musunuru [ ] (Addgene plasmid # 44719; http://n2t.net/addgene:44719; RRID: Addgene_44719). ..

    Expressing:

    Article Title: Bone Metastatic Progression of Prostate Cancer is Regulated by TRIM28-LDHA Mediated Metabolism
    Article Snippet: LDHA promoter luciferase reporter assays were conducted according to the manual of Dual-Luciferase Reporter Assay System from Promega. .. Briefly, C4–2B cells were seeded in a 24-well plate and co-transfected with the Renilla expression plasmid pRL-TK (RRID:Addgene_27163) and the reporter constructs for LDHA promoter in pGL3 vector (RRID:Addgene_212937). ..

    Construct:

    Article Title: Bone Metastatic Progression of Prostate Cancer is Regulated by TRIM28-LDHA Mediated Metabolism
    Article Snippet: LDHA promoter luciferase reporter assays were conducted according to the manual of Dual-Luciferase Reporter Assay System from Promega. .. Briefly, C4–2B cells were seeded in a 24-well plate and co-transfected with the Renilla expression plasmid pRL-TK (RRID:Addgene_27163) and the reporter constructs for LDHA promoter in pGL3 vector (RRID:Addgene_212937). ..

    Article Title: Nuclear GRP78 Promotes Metabolic Reprogramming and Therapeutic Resistance in Pancreatic Ductal Adenocarcinoma
    Article Snippet: Purpose: Stromal fibrosis limits nutritional supply and disarrays metabolism in pancreatic cancer (PDA, pancreatic ductal adenocarcinoma).. Understanding of themolecular basis underlyingmetabolic cues would improve PDA management.. The current study determined the interactionbetweenglucose-regulatedproteins78(GRP78) and hypoxia-inducible factor 1a (HIF-1a) and its mechanistic roles underlying PDA response to oxygen and glucose restrains.

    Article Title: Oncogenic KRAS Drives Lipofibrogenesis to Promote Angiogenesis and Colon Cancer Progression
    Article Snippet: DECEMBER 2023 CANCER DISCOVERY | 2653

    Clone Assay:

    Article Title: Nuclear GRP78 Promotes Metabolic Reprogramming and Therapeutic Resistance in Pancreatic Ductal Adenocarcinoma
    Article Snippet: Purpose: Stromal fibrosis limits nutritional supply and disarrays metabolism in pancreatic cancer (PDA, pancreatic ductal adenocarcinoma).. Understanding of themolecular basis underlyingmetabolic cues would improve PDA management.. The current study determined the interactionbetweenglucose-regulatedproteins78(GRP78) and hypoxia-inducible factor 1a (HIF-1a) and its mechanistic roles underlying PDA response to oxygen and glucose restrains.

    Amplification:

    Article Title: Oncogenic KRAS Drives Lipofibrogenesis to Promote Angiogenesis and Colon Cancer Progression
    Article Snippet: DECEMBER 2023 CANCER DISCOVERY | 2653

    Polymerase Chain Reaction:

    Article Title: Oncogenic KRAS Drives Lipofibrogenesis to Promote Angiogenesis and Colon Cancer Progression
    Article Snippet: DECEMBER 2023 CANCER DISCOVERY | 2653



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